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Coimmunoprecipitation of yotiao and NR1. Lysates of COS-7 cells doubly transfected with cDNA expression constructs encoding yotiao and NR1A were immunoprecipitated with antibodies against yotiao, NR1, or Kv1.4. Immunoprecipitates were then immunoblotted for yotiao and NR1 as indicated. Input lane contains 10% of the cell lysate used for the immunoprecipitation reaction. Positions of molecular weight markers are indicated in kilodaltons. Yt, Yotiao.
