Fig. 2.

Effect of AMPH (10 μm) on stimulated release and baseline overflow of DA in the absence and presence of sulpiride (2 μm), a D2 autoreceptor antagonist. Stimulated DA release is measured by cyclic voltammetry as the peak height of DA elicited by a single electrical pulse, and baseline overflow is measured as the average DA concentration change over a 5 min period between stimulations. Filled barsare wild-type data, and open bars are homozygote DAT knockout data. AMPH (top) or AMPH + sulpiride (AMPH + SULP) (bottom) were added to the slice 5 min before the first measurements. Results are the mean ± SEM of at least four independent experiments. *Significantly different from AMPH alone data (p < 0.05).