Fig. 1.
Effects of LPS (1 mg/kg) and perfusion on levels of IL-1β protein in the posterior cortex, hippocampus, and hypothalamus as measured by ELISA using the anti-rat IL-1β Ab from Dr. S. Poole. Eleven rats received an intraperitoneal injection of sterile 0.9% saline as controls, whereas another 11 rats received an intraperitoneal injection of LPS. From these groups, six of the animals were perfused with 0.9% saline before tissue collection. All animals were killed at 7 hr after injections. Saline nonperfused,n = 5; saline perfused, n = 6; LPS nonperfused, n = 5; LPS perfused,n = 6.