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. 1998 Mar 15;18(6):1987–1995. doi: 10.1523/JNEUROSCI.18-06-01987.1998

Fig. 4.

Fig. 4.

Progress curves of ATP and ATP analog catabolism in CA1 hippocampal slices and synaptosomes. ATP or ATP analogs (30 μm) were incubated at zero time with CA1 slices or synaptosomes. Samples (50 μl) were collected from the bath (600 μl) at the times indicated in the abscissa and analyzed by HPLC. InA is shown the catabolism of ATP (•) into ADP (▪), AMP (▴), and adenosine (▾) in CA1 slices. In B is shown the catabolism of ATP (○) into ADP (□), AMP (▵), and adenosine (▿) in CA1 synaptosomes. In C is shown the catabolism of ATPγS (•, ○) into AMP (▴,▵) in CA1 slices (filled symbols, filled lines) and CA1 synaptosomes (open symbols, broken lines). InD is shown the catabolism of β,γ-imido-ATP (•, ○) into AMP (▴,▵) in CA1 slices (filled symbols, filled lines) and CA1 synaptosomes (open symbols, broken lines). In E is shown the catabolism of β,γ-methylene-ATP (•, ○) into AMP (▴,▵) in CA1 slices (filled symbols, filled lines) and CA1 synaptosomes (open symbols, broken lines). InF is shown the catabolism of α,β-methylene ATP (•, ○) into α,β-methylene ADP (♦, ⋄) in CA1 slices (filled symbols, filled lines) and CA1 synaptosomes (open symbols, broken lines). InG is shown the catabolism of 2-methylthio-ATP (•) into 2-methylthio-ADP (▪), 2-methylthio-AMP (▴) and into an unidentified compound (▾) in CA1 slices. In H is shown the catabolism of 2-methylthio-ATP (○) into 2-methylthio-ADP (□), 2-methylthio-AMP (▵) and into an unidentified compound (▿) in CA1 synaptosomes. Each point is the average of four to five experiments. The vertical bars represent the SEM and are shown when they exceed the symbols in size. The concentrations of inosine and adenosine detected under control conditions, without addition of initial substrate, were subtracted. The concentration of any purine metabolite present at time 0 was also subtracted.