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. 1998 Sep 1;18(17):6631–6640. doi: 10.1523/JNEUROSCI.18-17-06631.1998

Fig. 5.

Fig. 5.

Simultaneous detection of POMC and POMC27* mRNA in different tissues of transgenic mice by an RNase protection strategy.A, Description of the RNase protection assay developed to detect simultaneously both POMC mRNA forms isolated from single tissues as described in Results. The white andblack squares denote the 30 bp heterologous oligonucleotide inserted in exon 3. The black squaresdepict the 15-nucleotide portion that hybridizes to the riboprobe.B, Cytoplasmatic RNA samples isolated from different tissues of POMC27* transgenic and wild-type (WT) mice were subjected to an RNase protection assay using a mouse POMC* exon 3 riboprobe. A mouse β-actin riboprobe was included in all protection reactions as an internal control. Pit, Pituitary; AL, anterior lobe; BS, brainstem; RB, rest of brain; BC, brain cortex; Hy, hypothalamus; Cer, cerebellum; Test, testis; Ser, serum;Ova, ovary; Spl, spleen.