Table 3.
Oxidative stress induces metabotropic glutamate receptors
| Cells | Antibody | Condition | Time (hr) | ||||
|---|---|---|---|---|---|---|---|
| 0 | 2 | 4 | 6 | 8 | |||
| Primary | mGluR1 | −Cys | 1 ± 0.18 | 2.98 ± 0.35 | 8.79 ± 0.45 | 7.66 ± 0.26 | 13.2 ± 1.42 |
| Primary | mGluR1 | +Glu | 1 ± 0.15 | 3.81 ± 0.47 | 2.81 ± 0.34 | 15.6 ± 1.68 | 10.9 ± 1.17 |
| HT22 | mGluR5 | −Cys | 1 ± 0.21 | 2.64 ± 0.11 | 9.05 ± 1.42 | 15.8 ± 2.56 | 14.4 ± 2.26 |
| HT22 | mGluR5 | +Glu | 1 ± 0.17 | 2.61 ± 0.14 | 9.34 ± 0.56 | 12.6 ± 1.15 | 11.6 ± 1.18 |
The mGluRs were assayed after glutamate addition or growth in low cystine medium as described in Figure 2B. The density of the exposed film was quantitated and expressed as the relative increase (0 time = 1) ± SEM (n = 4).