Table 5.

Effects of LZα8AS on number and cell distribution at E9

Clonal cell distribution	LZα8AS (n = 103)	LZ14 (n = 65)	p value
Total clone	8.8  ± 1.0  (100%)	28.6  ± 2.4  (100%)	<0.0001
Tectal plate	1.8  ± 0.5  (20%)	14.8  ± 1.2  (52%)	<0.0001
Intermediate zone	1.1  ± 0.2  (12%)	3.6  ± 0.4  (13%)	<0.0001
Ventricular zone	6.0  ± 0.4  (68%)	10.1  ± 1.0  (35%)	<0.0001

Tectal ventricles were injected with LZα8AS or LZ14 on E3 (approximately stage 17), and tecta were analyzed at E9. For the vector LZα8AS, the number of clones used for analysis was 103 (n = 103); for the vector LZ14, the number of clones used for analysis was 65 (n = 65). The cell distribution of a mean cell number in each layer, ventricular zone, intermediate zone, and tectal plate, as well as mean total cells per clone are expressed as the mean cell number ± SEM. Significance of differences between control and experimental mean numbers of cells was assessed by the two-tailed t test.