Fig. 3.

Reduction of NR2A and NR2C subunit mRNA levels by BDNF. RNase protection assay of 25 μg of total RNA extracted from cerebellar granule cells exposed to serum-free medium in the absence (CT) or presence of BDNF (50 ng/ml) for 6 hr.DP, Digested probe; P, aliquot of the hybridization solution containing the cRNA probes for NR2A (R2A), NR2B (R2B), NR2C (R2C), and cyclophilin (cyc). Thecyc probe was labeled at a lower specific activity.M, Molecular weight markers (MspI-digested pBR322). Protected fragments (indicated by arrows) were visualized by overnight exposure on x-ray film.