Fig. 5.

K252a prevents the BDNF- and FGF2-mediated downregulation of NR2A expression. Cerebellar granule cells were preincubated with K252a (100 nm) 10 min before the addition of BDNF (50 ng/ml), FGF2 (50 ng/ml), or NMDA (100 μm). NR2A mRNA (A) and protein (B) levels were determined by RNase protection assay and Western blot analysis at 6 and 24 hr, respectively (NR2A protein levels in FGF2-treated cells were measured at 6 hr). Data, expressed as percent of control, are the mean ± SEM of three determinations from three separate cell preparations (n = 6). *p < 0.05 versus control;  p < 0.05 versus BDNF or FGF2.