Fig. 3.

Southern blot analysis of hybrid bass genomic DNA. Each lane contains 8 μg of genomic DNA digested withXbaI, NcoI, PstI,HindIII, or BamHI and resolved on an agarose gel. Molecular weights of HindIII digested λ phage DNA are shown as markers. A, Blot probed with the perch Cx35 intracellular loop fragment. A single band was labeled inlanes containing DNA digested with XbaI,NcoI, or HindIII, whereas two bands were labeled in lanes containing DNA digested withPstI or BamHI. B, Blot probed with the perch Cx34.7 intracellular loop fragment. A single band was labeled in each lane. The labeling of different bands in A and B indicate that different genes are involved, and suggest that both are single-copy genes. See Results for further details.