Fig. 4.

Northern blot analysis of connexin distribution in hybrid bass tissues. The blot contains 35 μg of total RNA from each of the tissues indicated. A, Hybridization with the Cx35 intracellular loop probe labeled a 4.0 kb transcript in retina and brain RNA and weakly labeled a 4.8 kb transcript in both tissues. Other tissues lacked detectable transcripts. B, The Cx34.7 intracellular loop probe labeled a 13 kb transcript in retina and brain RNA, with very weak labeling of a transcript of the same size in smooth muscle (gut) RNA. C, Labeling of the 28 S rRNA shows approximately equal loading of alllanes.