Table 2.
Perch connexins fail to form heterotypic channels with endogenous Xenopus Cx38
| Oocyte injection (cell 1/cell 2) | Gj(μS) | # of pairs |
|---|---|---|
| antisense/antisense | 0.02 ± 0.01 | 8 |
| Cx34.7/water | 0.02 ± 0.01 | 8 |
| Cx35/water | 0.05 ± 0.02 | 8 |
| Cx34.7/Cx34.7 | 2.72 ± 0.35 | 5 |
| Cx35/Cx35 | 2.81 ± 1.10 | 3 |
Oocytes were either injected with an oligonucleotide antisense to a region within the coding sequence of Xenopus Cx38 (see Materials and Methods) or mock-treated (water), before receiving the specified cRNAs. The development of junctional conductance (Gj) was analyzed using a dual voltage-clamp procedure, and measurements of steady-state Gjwere performed 24–48 hr after pairing. Homotypic pairs expressing Cx34.7 and Cx35 were used as positive controls of cRNA injections and oocyte viability. Results are shown as mean ± SEM of the specified number of pairs.