Fig. 3.
Neuronal identity of newly divided cells in the dentate gyrus after global ischemia. BrdU was administered twice daily 9–12 d after 10 min of global ischemia. Gerbils were killed 4 weeks later, and brain sections were stained for BrdU immunoreactivity (green) and cell-specific markers (red). A, B, Colocalization of BrdU with calbindin-D28k(CalB) or NeuN is shown by yellow nuclei.C, Colocalization of BrdU with MAP-2 is shown in cells with red cytoplasm surrounding greennuclei. D, No colocalization of GFAP and BrdU was observed within the GCL. Scale bar, 10 μm.