Characterization of GLT-1 antibody.A, Immunoblot analysis using brain extracts from the adult wild-type (a), GLT-1(+/−) (b), and GLT-1(−/−) (c) mice. The size of protein markers is 205, 116, 80, and 49.5 kDa from the above. B, Immunohistochemistry for GLT-1 in the adult mouse brain. C, In situhybridization for GLT-1 mRNA in the adult mouse brain.D, Immunohistochemistry for GLT-1 in the adult mouse spinal cord. Cb, Cerebellum; CP, caudate–putamen; Cx, cerebral cortex;DH, dorsal horn; Di, diencephalon;Hi, hippocampus; Mb, midbrain;MO, medulla oblongata; Po, pons;Tu, olfactory tubercle; VH, ventral horn. Scale bars: B, C, 2 mm; D, 100 μm.