Table 1.
Mutations in eag specifically decreased the proportion of olfactory neurons responsive to the odorant EB
| Genotype | Response1-a to 10−6 EB (fold change compared with basal activity) | Response to 10−4 EB (fold change) | Response to 10−6 2-HEPT (fold change) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Increase1-b | Decrease | % (n) | Increase1-b | Decrease | % (n) | Increase1-b | Decrease | % (n) | |
| Control lines | |||||||||
| g eaghd15-Rev3 sd f | 3.2 ± 0.5 | 0.37 ± 0.06 | 47 | NT | NT | NT | NT | ||
| (n = 9) | (n = 5) | (n = 30) | |||||||
| CS | 4.0 ± 0.6 | 0.46 ± 0.06 | 52 | 7.5 ± 1.1 | 0.46 ± 0.15 | 47 | 3.1 ± 0.4 | 0.19 | 24 |
| (n = 31) | (n = 9) | (n = 79) | (n = 40) | (n = 4) | (n = 94) | (n = 14) | (n = 1) | (n = 53) | |
| Female controls from the eagX6stock | 6.4 ± 2.5 | 0.57 ± 0.05 | 50 | 6.7 ± 1.6 | 0.29 ± 0.1 | 62 | NT | NT | |
| (n = 7) | (n = 2) | (n = 18) | (n = 19) | (n = 4) | (n = 37) | ||||
| eag mutant lines | |||||||||
| g eaghd15 sd f | 3.1 ± 0.4 | 0.46 | 25 | NT | NT | NT | NT | ||
| (n = 6) | (n = 1) | (n = 28) | |||||||
| eagsc29 | 3.4 ± 1.1 | 17 | 7.0 ± 1.4 | 0.4 ± 0.07 | 39 | NT | NT | ||
| (n = 2) | (n = 0) | (n = 12) | (n = 17) | (n = 4) | (n = 54) | ||||
| eag1 | 2.7 ± 0.3 | 0.35 ± 0.05 | 23 | 5.0 ± 0.7 | 0.14 | 39 | 4.7 ± 0.8 | 0.55 ± 0.12 | 38 |
| (n = 13) | (n = 3) | (n = 52) | (n = 12) | (n = 1) | (n = 33) | (n = 20) | (n = 2) | (n = 58) | |
| eagX6 | 2.9 ± 0.7 | 21 | 7.8 ± 2.3 | 35 | NT | NT | |||
| (n = 4) | (n = 0) | (n = 19) | (n = 9) | (n = 0) | (n = 26) | ||||
Mutations in eag specifically decreased the proportion of olfactory neurons responsive to the odorant EB. Odorant-induced modulation of basal activity was determined during exposure of control and eag antennae to EB (10−6 and 10−4) dilutions) and 2-HEPT (10−6 dilution) using the loose patch recording technique. No significant difference in the magnitude of these odorant responses was observed among genotypes (Student’s t test).
Values indicate the action potential frequency during the odorant response (usually >1 sec) divided by the apparent spontaneous frequency.
Values indicate the fold-increase over basal levels for tonic and phasic–tonic responses (see Materials and Methods). Phasic–tonic responses were observed most often after 10−4 EB application in similar proportions of neurons from control and eag alleles (controls, 15%;eag alleles, 23%). NT, Not tested.