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The message for chick APP shows increased expression in motoneurons deprived of MEx in vitro. RNA was collected from motoneurons with (+) or without (−) MEx after 24 hr in culture. A, The Northern blot was probed with32P-labeled clone 22–2a (chick APP) under high stringency conditions, and the 2.3 kbp message was detected (arrow). B, The ethidium bromide-stained RNA gel before transfer to membrane with the 18 S RNA is indicated (arrow).
