Estrogen receptors are found in GAD-positive neurons of rat hippocampal cultures. A,B, Double staining of GAD immunoreactivity (red) and estrogen receptor (green) produce orange fluorescing cells.C, An estrogen receptor-negative pyramidal neuron surrounded by GAD-positive puncta, staining GABAergic terminals on the soma. Scale bar, 20 μm. Note GAD-positive terminals on the somata of the estrogen receptor-positive neurons, indicating that ERαs are confined to the somata and do not travel to the GAD-positive terminals.