Fig. 6.

Coexpression with dSLIP1 does not affect the single-channel conductance or the open probability of hSlo channels.A, Traces (1 sec) of steady-state recordings from patches excised from an oocyte expressing hSlo (left) or hSlo and dSLIP1 (right). Patches were exposed to 100 μm free Ca²⁺ and held at +60 mV (top) or −40 mV (bottom). Both patches contained two channels; the closed levels are indicated on theleft. B, Single-channel amplitudes as a function of voltage in two patches containing two channels (filled circles, same patch as A) or a single-channel (open squares) from an oocyte expressing hSlo or hSlo and dSLIP1, respectively. Amplitudes were determined by fitting gaussian distributions to amplitude histograms representing at least 1000 events. Linear regression analysis yielded single-channel conductances of 227 pS for hSlo and 226 pS for hSlo and dSLIP1. C, Single-channel open probability as a function of voltage for the two patches in B.