Fig. 1.

Enhancement of adenovirus infectivity in vivo by hypertonic mannitol. AxCALacZ (6.3 × 10⁷ pfu/5 μl) suspended in 1 mmannitol (A, C, E, G) or in isotonic PBS (B, D, F, H) was stereotactically injected into the left dorsal hippocampus. Five days later, infected cells were histochemically detected for β-gal activity (X-gal staining). A, B, The dentate gyrus of the hippocampus; C, D, the pyramidal cell layer of the CA3 region; E, F, the perforant pathway; G, H, the ipsilateral entorhinal cortex (layer II). Arrows point to the regions above for orientation. Scale bar (shown in H forA–H): 200 μm.