Fig. 3.

Sections of a brain 3 d after ischemia hybridized with ³⁵S-Ptn antisense cRNA (A, B) and immunostaining with anti-PTN antibody (C) or anti-GFAP antibody (D). Ptn hybridization signal was predominantly expressed in the neurons (large arrow) and glial cells (small arrow) along the border of the infarct and blood vessels (arrowhead) immediately adjacent to the infarct (top right in A, dark field, B, bright field). PTN immunoreactivity was detected in glial cells (large arrow) at the border of the infarct (C), which corresponded to the hypertrophic anti-GFAP(+) astrocytes in the similar area on day 3 (D, large arrow); the small arrow denotes neurons under degeneration. Magnification, 200×.