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Sections of an ischemic brain on day 3. A, B, PTN immunoreactivity was found in endothelial cells (small arrow) and macrophages (large arrow) in the infarcted region after staining with anti-PTN antibody. C, D, Macrophages surrounding blood vessel (large arrow) were identified by immunostaining with anti-OX42 antibody (C, arrow, frozen section) and histochemistry staining with GSA-IB4(D, arrow). Magnification: A, B, 400×;C, D, 200×.
