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. 1998 May 15;18(10):3620–3629. doi: 10.1523/JNEUROSCI.18-10-03620.1998

Fig. 6.

Fig. 6.

Blocking MAPK activation causes a delay in astrocytic differentiation. a, MAPKK inhibitor PD098059 was used to block MAPK activation. Cells were treated with CNTF for 24 or 48 hr in the presence or absence of 10 or 20 μmPD098059, fixed with 4% paraformaldehyde, and stained for GFAP. For the 48 hr time points, cells were retreated with CNTF and PD098059 at 24 hr. A representative experiment of the data plotted inb is shown. A–D, I–L, Immunofluorescent staining of GFAP. E–H,M–P, Corresponding bright-field (phase) images of these fields, E being the same field as A and so on. A–H, Images of cells fixed 24 hr after treatment and I–P at 48 hr after treatment. Scale bar:P, 100 μm. b, Average percentage of GFAP-positive cells was plotted for each condition.Squares, 24 hr time points; diamonds, 48 hr time points. Astrocytic differentiation is prevented by PD098059 only at the 24 hr time point.