Table 1.
Activated MAPKK causes a transient expression of GFAP in the absence of CNTF treatment
| Condition | MOI | No. of GFAP-positive cells/40 × field | |
|---|---|---|---|
| 24 hr | 48 hr | ||
| Experiment 1 | |||
| Mock | 0.67 | 0.1 | |
| WT | 50 | 0.97 | 5.5 |
| MAPKK | 50 | 11.0 | 1.66 |
| Experiment 2 | |||
| Mock | 2.5 | 0 | |
| WT | 50 | 2.3 | 0 |
| MAPKK | 50 | 8.75 | 0.05 |
Cells were infected with either WT adenovirus or an adenovirus vector expressing a constitutively active form of MAPKK at a multiplicity of infection (MOI) of ∼50. Cells were fixed with 4% paraformaldehyde 24 or 48 hr after infection and stained for GFAP. The number of GFAP-positive cells per 40× field is shown. There is an 11.3-fold increase in GFAP expression at 24 hr after infection in experiment 1, and a 3.8-fold increase in experiment 2. When compared with a WT infection, there is no increase in GFAP expression at 48 hr after infection in an MAPKK infection.