Fig. 7.
Graphic representation of the density of μ-ORi fibers in the medial part of the MPN (MPNm) (solid line) and the posterodorsal medial amygdaloid nucleus (MeApd) (dashed line) after estrogen treatment. The moment of EB injections is time 0. The density of distinct μ-OR+ fibers was significantly elevated by 30 min and peaks at 4–6 hr. The density of μ-OR+ fibers is basal by 48 hr. The bars at the 4 hr time point illustrate the density of μ-OR+ fibers in the MPNm (solid bar) and MeApd (open bar) in OVX plus EB rats pretreated with naltrexone 24 and 4 hr before perfusion. The density of μ-OR+ fibers in the naltrexone-treated rats is significantly lower than in ovariectomized rats in the MPN and MeApd. Each point is the mean ± SEM (n = 4 per time point).