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Histogram showing [3H]DAMGO binding in the MeA (solid bar) and MPO (open bar) at 0, 6, 12, and 24 hr after subcutaneous injection of 50 μg of EB. No significant change in [3H]DAMGO binding was observed in either the MeA or MPO after estrogen injection. Each point is the mean ± SEM (n = 4 per time point).
