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. 1998 May 15;18(10):3779–3785. doi: 10.1523/JNEUROSCI.18-10-03779.1998

Fig. 1.

Fig. 1.

Color-coded autoradiograms of coronal brainstem sections from the same rat show total [35S]GTPγS binding for three different treatment conditions: carbachol (1 mm), basal, and carbachol in the presence of atropine (1 mm). Color bar, Total [35S]GTPγS binding in nanocuries per gram. Adjacent sections from bregma −8.30 mm (top) and bregma −9.50 mm (bottom) show carbachol-stimulated G-protein activation in specific REM sleep-related nuclei (DR, PPT, PnO, LDT, and PnC) and in several non-sleep-related nuclei (IC, CnF, VTg, Pn, and Mo5). In all nuclei, this activation was blocked by the muscarinic antagonist atropine, confirming that carbachol-induced G-protein activation was mediated by mAChRs. CnF, Cuneiform nucleus;DR, dorsal raphe nucleus; IC, inferior colliculus; LC, locus coeruleus; LDT, laterodorsal tegmental nucleus; Mo5, motor trigeminal nucleus; Pn, pontine nuclei; PnC, nucleus pontis caudalis; PnO, nucleus pontis oralis;PPT, pedunculopontine tegmental nucleus;VTg, ventral tegmental nucleus.