Fig. 2.

Quantitative analysis of carbachol-stimulated G-protein activation in sleep-related brainstem nuclei from six animals. A, Bars represent mean specific [³⁵S]GTPγS binding + SEM for each of the treatment conditions: basal binding assayed without agonist and carbachol-induced [³⁵S]GTPγS binding assayed in the presence of 1 mm carbachol with or without atropine.B, Rank ordering of carbachol-stimulated [³⁵S]GTPγS binding. Results are reported as percent increase from basal [³⁵S]GTPγS binding. *Statistically significant increase in G-protein activation over basal levels (p < 0.05).