Fig. 2.

Time course of AMPA-induced neuronal death at pH 7.4 and 6.6. Sister cultures were incubated in HBSS containing 300 μm AMPA and 10 μm MK-801 for the indicated periods. LDH release to the bathing medium was measured 24 hr later (mean ± SEM, n = 4) and scaled to the level (100) measured in sister cultures exposed to 300 μm NMDA for 24 hr (a condition that produced near complete neuronal death without astrocyte death). Background LDH release in sister cultures exposed to sham wash alone was subtracted from each condition to yield the signal specific to experimental injury. *Difference at p < 0.05, from corresponding time value at pH 7.4, by Student’s two-tailed independent t test.