Fig. 4.

Acidic pH exacerbation of AMPA toxicity is associated with reduced ⁴⁵Ca uptake. Mixed cultures were incubated with 300 μm AMPA plus 100 μmcyclothiazide and 10 μm MK-801 at pH 7.4 or 6.6, for the indicated times. Cellular ⁴⁵Ca accumulation (mean ± SEM, n = 7) was scaled to the levels obtained in sister cultures exposed to 500 μm NMDA for 15 min (100). Basal ⁴⁵Ca uptake in sham-washed sister cultures was subtracted from all measurements. *Difference atp < 0.05, from corresponding time point at pH 7.4, by one-way ANOVA and Student Newman–Keuls test.