Fig. 7.

Mutational analysis of the effect of pervanadate on Kv1.3 expressed in HEK 293 cells. A, Schematic representation of the positions of the tyrosine residues in Kv1.3 that were mutated to phenylalanines. B–F, Kv1.3 outward currents evoked in cell-attached membrane patches by depolarizations from −80 to +40 mV, 20 min after addition of carrier (Control) or 100 μm sodium pervanadate (+Pervanadate) to the bath. The particular mutant channel analyzed is indicated in each panel (WT, wild-type).