Table 1.

Properties and modulation by pervanadate of wild-type and mutant Kv1.3 channels

Channel construct and treatment	Peak current (pA)	Inactivation time constant (msec)	Deactivation time constant (msec)
Wild-type Kv1.3 (n = 10)
Control	374  ± 69	861  ± 117	30  ± 4
Pervanadate	210  ± 40*	813  ± 73	34  ± 9
Y449F Kv1.3 (n = 6)
Control	491  ± 85	833  ± 72	37  ± 5
Pervanadate	466  ± 79	758  ± 79	63  ± 24
YYY111-113FFF Kv1.3 (n = 4)
Control	477  ± 120	775  ± 83	21  ± 4
Pervanadate	408  ± 86	677  ± 87	14  ± 3
Y137F Kv1.3 (n = 8)
Control	494  ± 134	838  ± 113	30  ± 3
Pervanadate	214  ± 90*	521  ± 91*	12  ± 3*
Y479F Kv1.3 (n = 5)
Control	837  ± 252	789  ± 161	33  ± 7
Pervanadate	533  ± 115*	683  ± 76	25  ± 4

Currents were evoked by 1 sec depolarizing pulses to +40 mV. Pervanadate treatment was as described for Figure 7. Values are means ± SEM. Time constant values were estimated from exponential fits to the inactivating or deactivating portion of the current. There were no significant differences (ANOVA) in basal biophysical parameters of the wild-type and mutant channels.

*Pervanadate-treated is significantly different from control by pairedt test.