Table 2.

Properties and modulation by insulin of wild-type and mutant Kv1.3 channels

Channel construct and treatment	Peak current (pA)	Inactivation time constant (msec)	Deactivation time constant (msec)
Wild-type Kv1.3 (n = 14)
Control	1411  ± 289	711  ± 52	34  ± 5
Insulin	1195  ± 293*	616  ± 75	42  ± 9
Y449F Kv1.3 (n = 6)
Control	823  ± 220	736  ± 92	27  ± 4
Insulin	727  ± 227*	621  ± 67	26  ± 7
YYY111-113FFF Kv1.3 (n = 4)
Control	483  ± 139	725  ± 121	34  ± 3
Insulin	444  ± 121	636  ± 87	43  ± 8
Y137F Kv1.3 (n = 6)
Control	1186  ± 467	788  ± 45	25  ± 3
Insulin	1081  ± 454	715  ± 109	41  ± 5*
Y479F Kv1.3 (n = 6)
Control	2167  ± 303	642  ± 70	31  ± 4
Insulin	2190  ± 412	578  ± 78	34  ± 5

Cells were transfected with both Kv1.3 and the IR. Currents were evoked by 1 sec depolarizing pulses to +40 mV. Insulin treatment was as described for Figure 10. Values are means ± SEM. Time constant values were estimated from exponential fits to the inactivating or deactivating portion of the current.

*Insulin-treated is significantly different from control by pairedt test.