Table 2.

Effects of various nucleotides and nucleotide analogs on calcineurin phosphatase activity

	OD
Condition	Mean    SD
3 mM sodium pyrophosphate + 0.3 mM ATP	0.02*	0.03
3 mM AMP-PNP + 0.3 mM ATP	0.23*	0.10
3 mM ADP + 0.3 mM ATP	0.30*	0.09
0.3 mM ATP	0.54	0.17
0.3 mM ADP + 0.3 mM ATP	0.60	0.35
1 mM AMP-PNP + 0.3 mM ATP	0.95*	0.27

A one-way ANOVA showed significant effects of the various solutions on calcineurin activity (p < 0.001). Results grouped within the ruled area are not significantly different from one another.

* indicates significantly different from 0.3 mM ATP.

n = 12 for each point.

The assay was repeated twice with six wells under each condition.