Table 2.
Depolarization of mitochondrial membrane after glutamate stimulation
| Glutamate concentration (nm) | Diploid | Ts16 | ||||||
|---|---|---|---|---|---|---|---|---|
| Group I (≤6 DIV) | Group II (7–12 DIV) | Group III (13–18 DIV) | Group IV (≥19 DIV) | Group I (≤6 DIV) | Group II (7–12 DIV) | Group III (13–18 DIV) | Group IV (≥19 DIV) | |
| 10 | 1.41 ± 0.20 | 1.29 ± 0.22 | 1.95 ± 0.25 | 2.20 ± 0.27 | 1.35 ± 0.22 | 1.39 ± 0.24 | 1.97 ± 0.27 | 2.55 ± 0.30 |
| 25 | 1.58 ± 0.24 | 1.98 ± 0.27 | 3.08 ± 0.30 | 3.08 ± 0.33 | 1.38 ± 0.23 | 2.37 ± 0.26 | 3.80 ± 0.29 | 4.35 ± 0.32** |
| 50 | 1.52 ± 0.20 | 2.77 ± 0.22 | 3.97 ± 0.25 | 4.22 ± 0.27 | 1.38 ± 0.25 | 3.03 ± 0.28 | 4.90 ± 0.31* | 5.49 ± 0.34** |
| 75 | 1.63 ± 0.25 | 3.20 ± 0.29 | 4.34 ± 0.32 | 4.80 ± 0.35 | 1.41 ± 0.17 | 3.52 ± 0.19 | 5.41 ± 0.21** | 6.11 ± 0.23** |
| 100 | 1.72 ± 0.19 | 3.44 ± 0.22 | 4.61 ± 0.24 | 5.20 ± 0.27 | 1.36 ± 0.21 | 3.62 ± 0.23 | 5.62 ± 0.26** | 6.43 ± 0.28** |
| 500 | 2.49 ± 0.14 | 4.26 ± 0.15 | 5.56 ± 0.17 | 5.56 ± 0.19 | 2.26 ± 0.20 | 4.40 ± 0.22 | 6.11 ± 0.24 | 6.80 ± 0.27** |
| 1000 | 2.57 ± 0.24 | 4.34 ± 0.27 | 5.80 ± 0.30 | 5.60 ± 0.33 | 2.37 ± 0.21 | 4.53 ± 0.23 | 6.16 ± 0.26 | 6.80 ± 0.28* |
After incubation with Rh123 (10 μg/ml), diploid and Ts16 hippocampal cultures of all age groups were stimulated with glutamate for 30 sec. In the table, the maximum Rh123 increase, given in ΔF/F0, indicates the amount of mitochondrial membrane depolarization after glutamate-induced [Ca2+]i increase. The data represent results with glutamate concentrations between 10 nm and 1 mm for all age groups out of three different preparations. Asterisks represent statistical significance between diploid and Ts16 data within each age group (*p < 0.05; **p < 0.01).