Fig. 5.

NSIST-transfected COS cells show different staining distributions for NSIST and mAb 3B3. Living NSIST-transfected COS cells were incubated with mAb 3B3, then fixed, permeabilized and double labeled with anti-NSIST antiserum RG211. Two distinct staining patterns emerge, with mAb 3B3 labeling the transfected cell surfaces in a punctate manner (a) and RG211 showing bright internal staining of only a subset of the mAb-3B3-positive cells (b). Transfected cells labeled with nonimmune serum show no staining (data not shown). Scale bar, 20 μm.