Fig. 11.
Expression of synaptic-vesicle proteins by hippocampal neurons in culture. Cells were grown for 14 d in control (A, C, E) or BDNF plus NT-3 conditions (B, D,F). Then, they were fixed and immunostained for the expression of synapsin-I (A,B). Different cultures were immunostained for the expression of synaptophysin (C, D), and SV2 (E, F). Magnification, 63×. No appreciable differences in the expression of the synaptic proteins were produced by the addition of BDNF plus NT-3 to the cultures. The same pattern was obtained after staining cultures from three experiments. Scale bar, 15 μm.