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Expression of TrkB and TrkC by E16 hippocampal neurons in culture. E16 cells grown for 14 d were fixed and immunostained for the expression of TrkB (A), TrkC (C), and GAD (B,D). Photographs were taken using a 40× objective. At least 95% of the neurons showed expression of TrkB and TrkC. TrkB and TrkC were present in both excitatory and inhibitory neurons. The same patterns were obtained after staining cultures from three experiments. Scale bars: A, B, 20 μm;C, D, 15 μm.
