Fig. 14.

Expression of synaptic-vesicle proteins and glutamate receptors by hippocampal neurons in culture. E16 hippocampal cells were grown on 6 or 10 cm dishes. After 11–13 d in vitro, proteins were analyzed by SDS-PAGE. Extracts were separated by electrophoresis on a 8% polyacrylamide gel and electrotransferred to nitrocellulose filters. Filters were immunoblotted with antibodies against synapsin-I (80–83 kDa), synaptotagmin (65 kDa), GluR2 (100 kDa), and GluR1 (100 kDa). The same pattern was obtained after analyzing extracts from two or three different cultures. Lane 1, Control; lane 2, chronic addition of BDNF and NT-3. There were not significant differences in the amount of these proteins between control and neurotrophin-treated cells (see Results).