Fig. 1.

Predicted organization of the Drosophila melanogaster FMRFamide prepropeptide. Left, The deduced dFMRFamide gene product is shown by avertical line with boxes to indicate the locations of several neuropeptide sequences. Center, Possible peptide intermediates were selected on the basis of the fact that each sequence was at least 70% identical to a sequence in a similar position in the Drosophila virilis FMRFamidegene (Taghert and Schneider, 1990). Right, Each of the eight peptides synthesized for this study is bordered by consensus sites for proteolytic cleavage from the precursor (Devi, 1991), contains a putative amidation signal at the C terminus (Bleakman et al., 1988), and contains two or more residues matching the FMRF consensus sequence common to this family of peptides (Greenberg and Price, 1992). *Purified from Drosophila tissue byNichols (1992); **purified from Drosophila tissue byNambu et al. (1988) and Nichols (1992). Hatched box, Signal sequence.