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Spinal motor neurons after 24 hr in culture.A, Phase-contrast micrograph. B, Immunofluorescence micrograph of the same field as in A, stained with the 4D5 anti-islet-1/2 monoclonal antibody. The SMNs were cultured in BDNF, CNTF, GDNF, HGF, forskolin, and IBMX. Scale bar, 50 μm.
