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. 1998 Dec 1;18(23):9733–9750. doi: 10.1523/JNEUROSCI.18-23-09733.1998

Fig. 11.

Fig. 11.

VGAT is associated with synaptic vesicles.A, Pre-embedding electron microscopic peroxidase immunocytochemistry suggests VGAT to be confined to the vesicle-rich areas (arrows) of the labeled terminals (T) and absent from the areas devoid of vesicles (stars), even when the latter are closer to the surface (s) of the Vibratome section (cerebellar nucleus with VGAT-containing terminals). B, C, The vesicular localization is confirmed by post-embedding immunogold labeling. The gold particles are restricted to vesicle-containing areas (arrows), whereas particles are rare in the rest of the terminal, containing neurofilaments (stars), mitochondria, or intervesicular cytoplasmic matrix. Note that at higher power (D), the plasma membrane appears clear of labeling, and that most of the particles are associated with the vesicles (basket cell terminals in cerebellar cortex).T, VGAT-labeled terminals; T′, VGAT-negative terminal; D, dendrites; A, myelinated axons; Pu, Purkinje cell body. Scale bars:A–C, 0.5 μm; D, 0.25 μm.