Fig. 4.

p174 derivatives expressed in the transgenic flies expressed normal levels of proteins and coimmunoprecipitated with calmodulin. A, Total head extracts fromninaC^P235 (ninaC null allele), Canton S (WT), and transgenic flies expressing the indicated p174 derivatives were fractionated by SDS-PAGE, and a Western blot was probed with αZB551 antiserum and¹²⁵I-labeled protein A. B, Coimmunoprecipitation of calmodulin with p174 from fly head extracts is shown. The p174 was immunoprecipitated with αp174 antiserum in the presence or absence of Ca²⁺, and a Western blot of the immune complexes was divided in half. Bottom, Probed with anti-calmodulin antibodies. Top, Probed with αZB551 antiserum.