Preservation of punctate staining for presynaptic terminals. Cultured hippocampal neurons were incubated in the absence (A, B) or presence (C,D) of 50 μm NMDA for 15 min before double-labeling for F-actin (A, C) or for the synaptic vesicle marker synapsin-1 (B,D). Even while F-actin hot spots were lost, punctate staining for nerve terminals was preserved. Scale bar, 10 μm.