Fig. 8.

Protective effect of the calcineurin antagonist ascomycin. Cultures were preincubated with 20 μm ascomycin (Asc) for 3 hr before treatment in the absence or presence of NMDA (50 μm; 5 min). The Ascalone group was not significantly different from control; theAsc+NMDA group was different from both control and NMDA-treated groups (p < 0.05, Tukey’s multiple comparisons test). Vertical bars indicate means; error bars indicate SEM (n = 7 neurons per group). Similar effects of ascomycin were observed in two additional independent experiments.