Fig. 3.

Distribution of hcrt-labeled neurons on frontal sections at three rostrocaudal levels of the tuberal region of the hypothalamus (A to B to C) determined using #2123 antiserum. Sections were counterstained with neutral red (pink staining of all cells). The neutral red staining allowed us to determine the exact location of hcrt neurons in the brain that are localized exclusively in the tuberal region of the hypothalamus ventral to the zona incerta and that extend 1 mm rostrocaudally, beginning caudal to the paraventricular nucleus of the hypothalamus. 3V, 3rd ventricle; Arc, arcuate nucleus; DMH, DM, dorsomedial hypothalamic nucleus; f, fornix; ic, internal capsule; opt, optic tract; SOR, retrochiasmatic part of the supraoptic nucleus; VMH, ventromedial hypothalamic nucleus; ZI, zona incerta. Scale bars, 275 μm.