Table 2.
Effect of divalent cations on cAMP production in HEK-293 cells stably transfected with AC9
| No divalents | 12.5 ± 0.5 | 
| Ca2+ | 9.9 ± 0.2* | 
| Ba2+ | 11.3 ± 0.4 | 
| Mg2+ | 12.7 ± 0.6 | 
| Sr2+ | 11.1 ± 0.7 | 
cAMP accumulation was evoked by the addition of 1 mmIBMX and 0.1 mm rolipram; data are expressed as multiples of the amount of cAMP found in cells not receiving PDE blockers, 80 ± 3 fmol/well in this experiment. Data are means ± SEM;n = 4 group. *p < 0.05 versus no divalents; one-way ANOVA, followed by Dunnett’s test.