Table 4.

The concentration of immunoreactive adenylyl cyclase 9 in selected regions of the rat brain and the anterior pituitary gland

Region	AC9-IR
Hippocampus	390  ± 15
Adenohypophysis	77  ± 2
Cerebellum	126  ± 7
Hypothalamus	257  ± 15

Membrane fractions prepared from dissected brain regions were extracted with RIPA buffer, and immunoreactive AC9 content (AC9-IR) was measured by radioimmunoassay with antiserum directed against the C-terminus of the protein, using the antigen peptide as the radiolabel and reference standard. Data are fmol/mg protein; mean ± SEM;n = 4–6/group.