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Assay as performed in Figure1A using neurons from the respective ganglia of chick E10 (A) and E12 (B) embryos. Data indicate that the GDNF/TGF-β synergism also applies to neurons at more advanced stages of development. Data are given as mean ± SEM (n = 6), p values derived from Student’s t test are ***p < 0.001 for increased survival as compared with single factors and +++p < 0.001 for decreased survival after antibody treatment.
