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RT-PCR (30 cycles) of P0 rat tissues and E14 purified motoneurons with primers specific for TGF-β2 (355 bp), TGF-β3 (290 bp), TβR-II (296 bp), GFRα-1 (286 bp), and c-ret (185 bp). PCR products obtained from rat ventral mesencephalon, DRG and purified motoneurons, and a 100 bp ladder are shown.Arrows indicate the size of the PCR products.
