Fig. 3.

Effects of mGluR₁ sense, mismatch, and antisense infusion on mGluR₁ and mGluR₅immunoreactivity in lumbar spinal cord.A–D show typical representations of mGluR₁ immunoreactivity in dorsal horn in control (saline), sense, mismatch, and antisense reagent-treated animals.E shows the virtual lack of immunoreactivity in control dorsal horn when the mGluR₁ antibody was preabsorbed with membranes from mGluR₁-overexpressing cells.F and G show mGluR₁immunoreactivity in ventral horn in control and mGluR₁antisense-treated animals. These results are typical of at least five animals in each case. Scale bars, 1.0 mm. H shows immunoblots using mGluR₁ and mGluR₅ antibodies after gel electrophoresis of lysates from spinal cord segments L3–L6 of (1) control, (2) antisense, (3) sense, and (4) mismatch-treated animals. The running positions of the molecular weight markers are shown in kilodaltons. Results are typical of three separate experiments.